Experiment equipment

BioWare Gene Gun

The BioWare gene gun is the first gene delivery system designed on the principle of a...

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BioWare Gene Gun

1. INTRODUCTION

The BioWare gene gun is the first gene delivery system designed based on the principle of aerodynamics in the world.
Its nozzles are designed to use lower pressure or cheaper gas to deliver various kind of biological materials into the target cells, tissues, animals and plants effectively in supersonic speed.
Comparing BioWare gene gun and the current products on the market, the latter is more expensive, can't be effectively clinicalized and must use gold particles as a carrier.
BioWare gene gun's manufacturing cost is relatively low and it is easier to use, and it is a quite competitive product of the same types of models.

The solution is atomized and accelerated to eject through the nozzle.

The high-speed continuous image of solution atomization at the nozzle outlet.

2. CHARACTeRISTICS

Lightweight and easy to operate is the most important characteristic of future gene guns for clinical treatment.
The invented apparatus implements miniaturization device with low cost.
The prototype of the gene gun developed by our company has completed vivo tests of animal and plant in laboratory.
Further development of product will give priority to foreign biotech instrument manufacturers or their subsidiaries in Taiwan as a partner for consultation or participation in development.

3. Application project

Currently the gene gun is mainly used in the following areas:

1) Transgenic plant

2) DNA vaccine
3) Gene therapy

4. Comparison between BioWare Gene Gun and Traditional Gene Gun

1) Characteristics of the BioWare gene gun:
(1) Does not cause damage to the tissue, and the sample damage rate is reduced
(2) Low pressure, low dose, low noise
(3) Lower price
(4) Helium can be replaced by nitrogen
(5) No need to use gold particles as a carrier
(6) The machine is light

2. Disadvantages of traditional models:
(1) Uneven distribution of particles, and damage to tissue
(2) The resulting sonic boom is unbearable for the test animal
(3) Machines are expensive
(4) The sample preparation process is complicated
(5) Small application range of delivery materials

Latest research results (please click)

Thepatents for gene gun has beenapplied in various countries.

National Innovation Award in 2002

International journal papers published

1. Cheng WF, Lee CN, Chang MC, Su YN, Chen CA, Hsieh CY. 2005. Antigen-specific CD8+ T lymphocytes generated from a DNA vaccine control tumors through the Fas-FasL pathway. Mol Ther. 12(5): 960-8.

2. Cheng WF, Hung CF, Chen CA, Lee CN, Su YN, Chai CY, Boyd DA, Hsieh CY, Wu TC. 2005. Characterization of DNA vaccines encoding the domains of calreticulin for their ability to elicit tumor-specific immunity and antiangiogenesis.Vaccine. 23(29): 3864-74.

3. Tu CF, Lin CC, Chen MC, Ko TM, Lin CM, Wang YC, Lai MD. 2007. Autologous neu DNA vaccine can be as effective as xenogenic neu DNA vaccine by altering administration route. Vaccine. 25(4):719-28.

4. Hsieh CY, Chen CA, Huang CY, Chang MC, Lee CN, Su YN, Cheng WF. 2007. IL-6-Encoding Tumor Antigen Generates Potent Cancer Immunotherapy Through Antigen Processing and Anti-apoptotic Pathways. Mol. Ther. 15(10) : 1890-7.

5. Bao-Wei Wang, Huei-Fong Hong, Hung Chang, Peiliang Kuan, and Kou-Gi Shyu. 2007. Mechanical stretch enhances the expression of resistin gene in cultured cardiomyocytes via tumor necrosis factor-α. Am J Physiol Heart Circ Physiol 293：H2305–H2312.

6. Chia-Yen Huang, Chi-An Chen, Chien-Nan Lee, Ming-Cheng Chang, Yi-Ning Su, Yi-Chun Lin, Chang-Yao Hsieh, Wen-Fang Cheng. 2007. DNA vaccine encoding heat shock protein 60 co-linked to HPV16 E6 and E7 tumor antigens generates more potent immunotherapeutic effects than respective E6 or E7 tumor antigens. Gynecologic Oncology 107(3):404-412.

7. Wen-Pin Cheng, Huei-Fong Hung, Bao-Wei Wang, and Kou-Gi Shyu. 2008. The molecular regulation of GADD153 in apoptosis of cultured vascular smooth muscle cells by cyclic mechanical stretch. Cardiovascular Research. 77 (3) : 551-559.

8. Lin CC, Yen MC, Lin CM, Huang SS, Yang HJ, Chow NH, Lai MD. 2008. Delivery of noncarrier naked DNA vaccine into the skin by supersonic flow induces a polarized T helper type 1 immune response to cancer. The Journal of Gene Medicine. 10(6):679-89.

9. Ming-Ling Chang, Jeng-Chang Chen, Chau-Ting Yeh, Ming-Yu Chang, Chun-Kai Liang, Cheng-Tang Chiu, Deng-Yn Lin, and Yun-Fan Liaw. 2008. Gene Gun Bombardment with DNA-Coated Gold Particles Is a Potential Alternative to Hydrodynamics-Based Transfection for Delivering Genes into Superficial Hepatocytes. HUMAN GENE THERAPY. 19:391–395.

10. Cheng WF, Chang MC, Sun WZ, Lee CN, Lin HW, Su YN, Hsieh CY and Chen CA. 2008. Connective tissue growth factor linked to the E7 tumor antigen generates potent antitumor immune responses mediated by an antiapoptotic mechanism. Gene Ther. Mar 20. 15 (13) : 1007-16.

11. Kao CY, Huang SH and Lin CM. 2008. A low-pressure gene gun for genetic transformation of maize (zea mays L.) Plant Biotechnol Rep. 2(4)：267-270.

12. Chen SH, Wang BW, Wang DL and Shyu KG. 2008 Hypoxia induce discoidin domain receptor-2 expression via the p38 pathway in vascular smooth muscle cell to increase their migration. Biochemica and Biophysical Research Communication. 374：662-667.

13. Wang BW, Chang H, Kuan P and Shyu KG. 2008. Angiotensin II actirates myostatin expression in cultured rat neonatal cardiomyocytes via p38 MAP kinase and myocyte enhance factor 2 pathway. Journal of Endocrinology. 197：85-93.

14. Hung HF., Wang BW., Chang H. and Shyu KG. 2008. The molecular regulation of resistin expression in cultured vascular smooth muscle cells under hypoxia. Journal of Hypertension 26:2349–2360

15. Lou PJ ., Cheng WF., Chung YC., Cheng CY., Chiu LH.,and Young TH. 2009. PMMA particle-mediated DNA vaccine for cervical cancer. Journal of Biomedical Materials Research Part A. 88A: 849–857.

16. Yen MC, Lin CC,Chen YL, Huang SS, Yang HJ, Chang CP, Lei HY, and Lai MD. 2009. A Novel Cancer Therapy by Skin Delivery of Indoleamine 2,3-Dioxygenase siRNA. Clin Cancer Res 15(2):641-649.

17. Lai MD, Yen MC, Lin CM, Tu CF, Wang CC, Lin PS, Huang HJ, and Lin CC. 2009. The effects of DNA formulation and administration route on cancer therapeutic efficacy with xenogenic EGFR DNA vaccine in a lung cancer animal model. Genet Vaccines Ther. 2009 7(1):2.

18. Chen CA, Chang MC, Sun WZ, Chen YL, Chiang YC, Hsieh CY, Chen SM, Hsiao PN, and Cheng WF. 2009. Noncarrier naked antigen-specific DNA vaccine generates potent antigen-specific immunologic responses and antitumor effects. Gene Therapy. (2009) 16, 776-787.

19. Lai MD, Chen CS,Yang CR,Yuan SY,Tsai JJ,Tu CF,Wang CC,Yen MC and Lin CC. An HDAC inhibitor enhances the antitumor activity of a CMV promoter-driven DNA vaccine. Cancer Gene Therapy (2009), 1–9.

20. Shyu KG, Chua SK, Wang BW and Kuan P. 2009. Mechanism of inhibitory effect of atorvastatin on resistin expression induced by tumor necrosis factor-α in macrophages. Journal of Biomedical Science 2009, 16:50.

21. Shyu KG, Wang BW and Chang H.2009. Hyperbaric oxygen activates discoidin domain receptor 2 via tumour necrosis factor-α and the p38 MAPK pathway to increase vascular smooth muscle cell migration through matrix metalloproteinase 2. Clinical Science 116, 575–583.

22. Cheng W. P., Wang B.W.and Shyu K. G. Regulation of GADD153 induced by mechanical stress in cardiomyocytes.ORIGINAL ARTICLE.Eur J Clin Invest 2009; 39 (11): 960–971.

23. Huang Chia-Hsin.Chang Chia-Che.Lin Chiu-Mei.Wang Sin-Ting.Wu Min-Tze.Li Eric I. C..Chang Hsien-Chang.Lin Chi-Chen.Promoting effect of Antrodia camphorata as an immunomodulating adjuvant on the antitumor efficacy of HER-2/neu DNA vaccine.Cancer Immunol Immunother.2010 March 17.

24. Huang CH, Chang CC, Lin CM, Wang ST, Wu MT, Li EIC, Chang HC, Lin CC. 2010. Promoting effect of Antrodia camphorate as an immunomodulating adjuvant on the antitumor efficacy of HER-2/neu DNA vaccine. Cancer Immunol Immunother 2010 Apr 14.

25. Wang BW, Chang H and Shyu KG. 2010. Regulation of resistin by cyclic mechanical stretch in cultured rat vascular smooth muscle cells. Clinical Science 118, 221–230.

26. Shyu KG, Wang BW, Chen WJ, Kuan P, and Hung CR. 2010. Mechanism of the inhibitory effect of atorvastatin on endoglin expression induced by transforming growth factor-β1 in cultured cardiac fibroblasts European Journal of Heart Failure 12, 219–226.

27. Shyu KG, Wang BW, Lin CM, Chang H. 2010. Cyclic stretch enhances the expression of Toll-like Receptor 4 gene in cultured cardiomyocytes via p38 MAP kinase and NF-κB pathway Journal of Biomedical Science 17:15..

28. Chen CA, Ho CM, Chang MC, Sun WZ, Chen YL, Chiang YC, Syu MH, Hsieh CY and Cheng WF. 2010. Metronomic Chemotherapy Enhances Antitumor Effects of Cancer Vaccine by Depleting Regulatory T Lymphocytes and Inhibiting Tumor. Angiogenesis. Mol Ther 2010 Apr 6.

29. Chiu Chiung-Zuan, Wang Bao-Wei, Chung Tun-Hui and Shyu Kou-Gi .Angiotensin II and the ERK pathway mediate the induction of myocardin by hypoxia in cultured rat neonatal cardiomyocytes.Clinical Science (2010) 119, 273–282

30. Wang S-T, Chang C-C, Yen M-C, Tu C-F, Chu C-L, Peng Y-T, Chen D-Y, Lan J-L and Lin C-C.RNA interference-mediated silencing of Foxo3 in antigen-presenting cells

as a strategy for the enhancement of DNA vaccine potency.ORIGINAL ARTICLE.Gene Therapy (2010),1–12

31. Lin Chi-Chen,Yu Yen-Ling,Shih Chia-Chiao,Liu Ko-Jiunn,Ou Keng-LiangHong Ling-Zong,Chen Jody D. C.,Chu Ching-Liang. A novel adjuvant Ling Zhi-8 enhances the eYcacy of DNA cancer vaccine by activating dendritic cells. ORIGINAL ARTICLE. Cancer Immunol Immunother(2011).

32. Shyu Kou-Gi,Wang Bao-Wei,Chen Wei-Jan,Kuan Peiliang,Lin Chiu-Mei.Angiotensin II mediates urotensin II expression by hypoxia in cultured cardiac

fibroblast.ORIGINAL ARTICLE.(2011).

33 Wang Bao-Wei,Lin Chiu-Mei,Wu Gong-Jhe,Shyu Kou-Gi.Tumor necrosis factor-α enhances hyperbaric oxygen-induced visfatin expression via JNK pathway in human coronary arterial endothelial cells.Journal of Biomedical Science 2011, 18:27

34. Huang Tzu-Ting, Yen Meng-Chi, Lin Chi-Chen, Weng Tzu-Yang, Chen Yi-Ling, Lin Chiu-Mei, Lai Ming-Derg. Skin delivery of short hairpin RNA of indoleamine 2,3 dioxygenase induces antitumor immunity against orthotopic and metastatic liver cancer. Cancer Science 2011; 102(12) : 2214-2220

35. Chiu Chiung-Zuan, Wang Bao- Wei, and Shyu Kou-Gi. Use of atorvastatin to inhibit hypoxia-induced myocardin expression.ORIGINAL ARTICLE. (2011)

36. Cheng Wen-Pin, Wang Bao-Wei, Chen Shih-Chung, Chang Hang, Shyu Kou-Gi. Mechanical stretch induces the apoptosis regulator PUMA in vascular smooth muscle cells. Cardiovascular Research 2012; 93: 181-189

37. http://www.nature.com/cgt/journal/vaop/ncurrent/full/cgt201327a.html

38. CZ Chiu, BW Wang, KG Shyu. Effects of cyclic stretch on the molecular regulation of myocardin in rat aortic vascular smooth muscle cells. Journal of Biomedical Science( 2013), 20:50.

39. TY Weng, SS Huang, MC Yen, CC Lin, YL Chen, CM Lin, WC Chen, CY Wang, JY Chang and MD Lai. A Novel Cancer Therapeutic Using Thrombospondin 1 in Dendritic Cells. Molecular Therapy (2013) doi:10.1038/mt.2013.236.

40. BW Wang, GJ Wu, WP Cheng, KG Shyu. Mechanical stretch via transforming growth factor-β1 activates microRNA-208a to regulate hypertrophy in cultured rat cardiac myocytes. Journal of the Formosan Medical Association (2013) Volume 112, Issue 10.

41. Wang BW, Wu GJ, Cheng WP, Shyu KG (2014) MicroRNA-208a Increases Myocardial Fibrosis via Endoglin in Volume Overloading Heart. PLoS ONE 9(1):e84188. doi:10.1371/journal.pone.0084188.
42. KG Shyu, WP Cheng, BW Wang and H Chang. .Hypoxia activates muscle-restricted coiled-coil protein (MURC)

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